BLK Kinase Assay Service

Activity-based BLK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BLK
Enzyme
Human BLK
HGNC
BLK
Synonym
BLK
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine2.1
PP2379
AG147812,000
D-644064,100
Scientific Information
P51451 (http://www.uniprot.org/uniprot/P51451)
Screening Location
Malvern, USA
Reference compound IC50 for BLK
Reference compound IC50 for BLK