BRAF-FAM131B Kinase Assay Service

Activity-based BRAF-FAM131B kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BRAF, FAM131B-
Enzyme
Human FAM131B-BRAF fusion
HGNC
BRAF-FAM131B
Synonym
B-Raf, B-raf-1, BRAF1, RAFB1
Substrates
MEK1 (K97R), 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
FAM131B: Q86XD5 (http://www.uniprot.org/uniprot/Q86XD5)BRAF: P15056 (http://www.uniprot.org/uniprot/P15056)
Screening Location
Malvern, USA
Reference compound IC50 for BRAF-FAM131B