BRAF-KIAA1549 (Kex16Bex9) Kinase Assay Service

Activity-based BRAF-KIAA1549 (Kex16Bex9) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BRAF, KIAA1549-
Enzyme
Human KIAA1549-BRAF fusion
HGNC
BRAF
Synonym
B-Raf, B-raf-1, BRAF1, RAFB1
Substrates
MEK1 (K97R), 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine13,000
Ro 31-822011,300
GW507413
H-89>100,000
Rottlerin>100,000
Scientific Information
KIAA1549: Q9HCM3 (http://www.uniprot.org/uniprot/Q9HCM3)BRAF: P15056 (http://www.uniprot.org/uniprot/P15056)
Screening Location
Malvern, USA
Reference compound IC50 for BRAF KIAA1549 (Kex16Bex9)
Reference compound IC50 for BRAF KIAA1549 (Kex16Bex9)