BRAF (G469A) Kinase Assay Service

Activity-based BRAF (G469A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BRAF (G469A)
Enzyme
Human BRAF (G469A)
HGNC
BRAF (G469A)
Synonym
B-Raf, B-raf-1, BRAF1, RAFB1
Substrates
MEK1 (K97R), 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine>100,000
Ro 31-822019,500
GW50748.0
H-89>100,000
Rottlerin>100,000
Scientific Information
P15056 (http://www.uniprot.org/uniprot/P15056)
Screening Location
Malvern, USA
Reference compound IC50 for BRAF (G469A)
Reference compound IC50 for BRAF (G469A)
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