Activity-based BRAF (V599E) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BRAF (V599E)
Enzyme
Human BRAF (V599E)
HGNC
BRAF (V600E)
Synonym
B-Raf, B-raf-1, BRAF1, RAFB1
Substrates
MEK1 (K97R), 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P15056 (http://www.uniprot.org/uniprot/P15056)
Screening Location
Malvern, USA
Further information
More information can be found on our website Kinase Assays.
Reference compound IC50 for BRAF (V599E)
