BRAF (V599E) Kinase Assay Service

Activity-based BRAF (V599E) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

BRAF (V599E)

Enzyme

Human BRAF (V599E)

HGNC

BRAF (V600E)

Synonym

B-Raf, B-raf-1, BRAF1, RAFB1

Substrates

MEK1 (K97R), 1 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
GW 5074 8.5
Scientific Information

P15056 (http://www.uniprot.org/uniprot/P15056)

Screening Location

Malvern, USA

Further information

More information can be found on our website Kinase Assays.

Reference compound IC50 for BRAF (V599E)

Reference compound IC50 for BRAF (V599E)