Activity-based BRAF V600E kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
BRAF V600E
Enzyme
Human BRAF V600E, mutant
HGNC
BRAF (V600E)
Manning ID
BRAF
Synonym
BRAF, B-Raf, B-raf-1, BRAF1, RAFB1, B-RAF V600E, BRAF V600E, BRAF (V599E)
UniProt
P15056 (BRAF_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
MEK1-K97M, ATP
Reaction
MEK1-K97M + 33P-ATP = 33P-MEK1-K97M + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Sorafenib | 660 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | BRAF V600E |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – BRAF V600E (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0404-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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