BRD1 Bromodomain Assay Service (ThermoShift)

BRD1-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview




Human BRD1 bromodomain, GST-tagged


BR140-like protein, BRL; Bromodomain and PHD finger-containing protein 2, BRPF-2

Assay Properties


Thermal stability change after ligand binding

Concentration of protein in assay

0.15 mg/ml (3.53 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)


Reference Compound ΔTms:

Bromosporine – 2.5 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

O95696 (

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak