BRD1 Bromodomain Assay Service (ThermoShift)

BRD1-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRD1-H

Protein

Human BRD1 bromodomain, His-tagged

Synonyms

BR140-like protein, BRL; Bromodomain and PHD finger-containing protein 2, BRPF-2

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.125 mg/ml (6.9 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

46

Reference Compound ΔTms:

Bromosporine - 3 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

O95696 (http://www.uniprot.org/uniprot/O95696)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak