BRD2-1 Bromodomain Assay Service (ThermoShift)

BRD2-1-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview

CAT#

Tm-BRD2-1-GST

Protein

Human BRD2-1 bromodomain, GST-tagged

Synonyms

O27.1.1, RING3

Assay Properties

Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.075 mg/ml (1.8 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

45

Reference Compound ΔTms:

JQ1 – 4 (Orange)PFI1 – 3.5 (Pink)BET151 – 4.5 (Blue)No stabilization was observed in the presence of other control compounds (CBP112, SGC-CBP30, RVX-208, GSK2801 and PFI3)

Scientific Information

P25440 (http://www.uniprot.org/uniprot/P25440)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak