BRD2-2 Bromodomain Assay Service (ThermoShift)

BRD2-2-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRD2-2-H

Protein

Human BRD2-2 bromodomain, His-tagged

Synonyms

O27.1.1, RING3

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.085 mg/ml (5.4 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

51,5

Reference Compound ΔTms:

JQ1 - 6.5 (Orange)PFI1 - 4 (Pink)BET151 - 6 (Blue)RVX-208 - 2.5 (Purple)No stabilization was observed in the presence of other control compounds (CBP112, GSK2801 and PFI3)

Scientific Information

P25440 (http://www.uniprot.org/uniprot/P25440)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak