BRD2 Bromodomain Assay Service (AlphaScreen)

BRD2-Tndm-His Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview
CAT#

BRD2-Tandem

Enzyme

Human BRD2-Tandem

Synonyms

Bromodomain-containing protein 2 domain 1 and domain 2, O27.1.1, Really interesting new gene 3 protein, KIAA9001, RING3

Substrates

Histone H4 peptide (1-21) K5/8/12/16Ac

Assay Properties
Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
JQ-1 75
PFI-1 1,200
Scientific Information

P25440 (http://www.uniprot.org/uniprot/P25440)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for BRD2-T

Reference Compound IC50 for BRD2-T