BRD4-1 Bromodomain Assay Service (ThermoShift)

BRD4-1-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRD4-1-H

Protein

Human BRD4-1 bromodomain, His-tagged

Synonyms

HUNK1

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.075 mg/ml (4.2 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

48

Reference Compound ΔTms:

JQ1 - 9.5 (Orange)PFI1 - 5.5 (Pink)BET151 - 9.5 (Blue)No stabilization was observed in the presence of other control compounds (CBP112, RVX-208, GSK2801 and PFI3)

Scientific Information

O60885 (http://www.uniprot.org/uniprot/O60885)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak