BRD4-2 Bromodomain Assay Service (ThermoShift)

BRD4-2-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRD4-2-GST

Protein

Human BRD4-2 bromodomain, GST-tagged

Synonyms

HUNK1

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.075 mg/ml (1.9 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

49,5

Reference Compound ΔTms:

JQ1 - 2 (Orange)PFI1 - 1 (Pink)BET151 - 1.5 (Blue)No stabilization was observed in the presence of other control compounds (CBP112, SGC-CBP30, GSK2801 and PFI3)

Scientific Information

O60885 (http://www.uniprot.org/uniprot/O60885)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak