BRD4 Bromodomain Assay Service (AlphaScreen)

BRD4 Full Length Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview
CAT#

BRD4 Full Length

Enzyme

Human BRD4 Full length

Synonyms

Bromodomain-containing protein 4 domain 1 and 2, Protein HUNK1

Substrates

Histone H4 peptide (1-21) K5/8/12/16Ac

Assay Properties
Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
JQ-1 50
PFI-1 385
Scientific Information

O60885 (http://www.uniprot.org/uniprot/O60885)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for BRD4 Full length

Reference Compound IC50 for BRD4 Full length