BRD4 Bromodomain Assay Service (ThermoShift)

BRD4 Full length Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRD4-FL-H

Protein

Human BRD4-FL bromodomain, His-tagged

Synonyms

HUNK1

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.2 mg/ml (1.3 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

48

Reference Compound ΔTms:

JQ1 - 9.5 (Orange)PFI1 - 4.5 (Pink)BET151 - 8 (Blue)RVX-208 - 1.5 (Purple)No stabilization was observed in the presence of other control compounds (CBP112, SGC-CBP30, GSK2801 and PFI3)

Scientific Information

O60885 (http://www.uniprot.org/uniprot/O60885)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak