BRD4-Tandem Bromodomain Assay Service (AlphaScreen)

BRD4-Tndm-His Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview
CAT#

BRD4-Tandem

Enzyme

Human BRD4-Tandem (domain 1 and 2)

Synonyms

Bromodomain-containing protein 4 domain 1 and 2, Protein HUNK1

Substrates

Histone H4 peptide (1-21) K5/8/12/16Ac

Assay Properties
Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
JQ-1 64
PFI-1 405
Scientific Information

O60885 (http://www.uniprot.org/uniprot/O60885)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for BRD4-Tandem

Reference Compound IC50 for BRD4-Tandem