BRD9 Bromodomain Assay Service (ThermoShift)

BRD9-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRD9-GST

Protein

Human BRD9 bromodomain, GST-tagged

Synonyms

Rhabdomyosarcoma antigen MU-RMS-40.8

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.2 mg/ml (4.95 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

43

Reference Compound ΔTms:

Bromosporine - 5.5 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, BET151, GSK2801 and PFI3)

Scientific Information

Scientific Information: Q9H8M2 (http://www.uniprot.org/uniprot/Q9H8M2)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak