BRDT-2-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BRDT-2-H
Protein
Human BRDT-2 bromodomain, His-tagged
Synonyms
CT9, RING3-like protein, BRD6
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.15 mg/ml (9.1 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
41
Reference Compound ΔTms:
JQ1 - 8.5 (Orange)PFI1 - 4.5 (Pink)BET151 - 4 (Blue)RVX-208 - 1 (Purple)No stabilization was observed in the presence of other control compounds (CBP112, SGC-CBP30, GSK2801 and PFI3)
Scientific Information
Q58F21 (http://www.uniprot.org/uniprot/Q58F21)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak