BRDT-2 Bromodomain Assay Service (ThermoShift)

BRDT-2-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview

CAT#

Tm-BRDT-2-H

Protein

Human BRDT-2 bromodomain, His-tagged

Synonyms

CT9, RING3-like protein, BRD6

Assay Properties

Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.15 mg/ml (9.1 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

41

Reference Compound ΔTms:

JQ1 – 8.5 (Orange)PFI1 – 4.5 (Pink)BET151 – 4 (Blue)RVX-208 – 1 (Purple)No stabilization was observed in the presence of other control compounds (CBP112, SGC-CBP30, GSK2801 and PFI3)

Scientific Information

Q58F21 (http://www.uniprot.org/uniprot/Q58F21)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak