BRDT Bromodomain Assay Service (AlphaScreen)

BRDT-Tndm-His Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview
CAT#

BRDT-Tandem

Enzyme

Human BRDT-Tandem

Synonyms

Bromodomain testis-specific protein domain 1 and domain 2, Cancer/testis antigen 9, RING3-like protein

Substrates

Histone H4 peptide (1-21) K5/8/12/16Ac

Assay Properties
Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
JQ-1 120
PFI-1 550
Scientific Information

Q58F21 (http://www.uniprot.org/uniprot/Q58F21)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for BRDT-T

Reference Compound IC50 for BRDT-T