BRK Kinase Assay Service

Activity-based BRK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BRK
Enzyme
Human BRK
HGNC
PTK6
Synonym
BRK
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q13882 (http://www.uniprot.org/uniprot/Q13882)
Screening Location
Malvern, USA
Reference compound IC50 for BRK