BRK Kinase Assay Service

Activity-based BRK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BRK
Enzyme
Human BRK
HGNC
PTK6
Synonym
BRK
Scientific Information
Q13882 (http://www.uniprot.org/uniprot/Q13882)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds IC50
PP2 10 nM
Staurosporine 285 nM
AG-1478 299.6 nM
D-64406 38.1 µM
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of BRK