BRPF1b Bromodomain Assay Service (ThermoShift)

BRPF1b-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview




Human BRPF1b bromodomain, GST-tagged


Peregrin; Bromodomain and PHD finger-containing protein 1; BR140

Assay Properties


Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.5µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)


Reference Compound ΔTms:

Bromosporine – 4.5 (Blue)PFI1 – 2 (Pink)RVX – 208 – 1.5 (Purple)No stabilization was observed in the presence of other control compounds (JQ1, CBP112, SGC-CBP30, BET151, GSK2801 and PFI3)

Scientific Information

P55201 (

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak