BRPF1b Bromodomain Assay Service (AlphaScreen)

BRPF1b-His Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview
CAT#

BRPF1b-His

Enzyme

Human BRPF1b-His

Synonyms

Bromodomain and PHD finger-containing protein 1, BR140

Substrates

Histone H4 peptide (1-21) K5/8/12/16Ac

Assay Properties
Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
JQ-1 65,000
PFI-1 2,100
Bromosporine 610
SGC-CBP30 5,700
GSK2801 18,000
Scientific Information

P55201 (http://www.uniprot.org/uniprot/P55201)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for BRPF1b

Reference Compound IC50 for BRPF1b