BRPF1b Bromodomain Assay Service (ThermoShift)

BRPF1b-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRPF1b-H

Protein

Human BRPF1b bromodomain, His-tagged

Synonyms

Peregrin; Bromodomain and PHD finger-containing protein 1; BR140

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.08 mg/ml (4.9 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

42

Reference Compound ΔTms:

Bromosporine - 6 (Blue)PFI1 - 2 (Pink)RVX - 208 - 2 (Purple)No stabilization was observed in the presence of other control compounds (JQ1, CBP112, SGC-CBP30, BET151, GSK2801 and PFI3)

Scientific Information

P55201 (http://www.uniprot.org/uniprot/P55201)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak