BRPF3 Bromodomain Assay Service (ThermoShift)

BRPF3-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRPF3-GST

Protein

Human BRPF3 bromodomain, GST-tagged

Synonyms

KIAA1286

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.15 mg/ml (3.69 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

43

Reference Compound ΔTms:

No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

Q9ULD4 (http://www.uniprot.org/uniprot/Q9ULD4)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak