BRWD3-2 Bromodomain Assay Service (ThermoShift)

BRWD3-2-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BRWD3-2-GST

Protein

Human BRWD3-2 bromodomain, GST-tagged

Synonyms

Bromodomain and WD repeat-containing protein 3

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.2 µM)

Concentration of compound in assay

25 M

Apo form Tm (°C)

49

Reference Compound ΔTms:

No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

Q6RI45 (http://www.uniprot.org/uniprot/Q6RI45)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak