BTK (E41K) Kinase Assay Service

Activity-based BTK (E41K) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
BTK (E41K)
Enzyme
Human BTK (E41K)
HGNC
BTK (E41K)
Synonym
AGMX1, AT, ATK, BPK, PSCTK1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine26.7
PP2428
AG147851,000
Scientific Information
Q06187 (http://www.uniprot.org/uniprot/Q06187)
Screening Location
Malvern, USA
Reference compound IC50 for BTK (E41K)
Reference compound IC50 for BTK (E41K)