BTK (T474I) Kinase Assay Service

Activity-based BTK (T474I) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

BTK (T474I)

Enzyme

Human BTK (T474I)

HGNC

BTK (T474I)

Synonym

Tyrosine-protein kinase BTK, Bruton tyrosine kinase, Agammaglobulinaemia tyrosine kinase, ATK, B cell progenitor kinase, BPK, AGMX1, AT, PSCTK1

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 9.3
PP2 3,000
AG1478 2,500
EGFR/ErbB Inhibitor 5.0
D-64406 287,000
Scientific Information

Q06187 (http://www.uniprot.org/uniprot/Q06187)

Screening Location

Malvern, USA

Reference compound IC50 for BTK (T474I)

Reference compound IC50 for BTK (T474I)