Activity-based c-KIT (d557-558/Y823D) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-Kit (d557-558/Y823D)
Enzyme
Human c-Kit (d557-558/Y823D)
HGNC
KIT (d557-558/Y823D)
Synonym
Mast/stem cell growth factor receptor, Proto-oncogene tyrosine-protein kinase c-kit
Scientific Information
P10721 (http://www.uniprot.org/uniprot/P10721)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.6 |
| PP2 | 5.9 |
| AG1478 | 400 |
| EGFR/ErbB Inhibitor | 17,000 |
| D-64406 | 960 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for c-Kit (d557-558/Y823D)
