c-KIT (V560G/D816V) Kinase Assay Service

Activity-based c-KIT (V560G/D816V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

c-KIT (V560G/D816V)

Enzyme

Human c-KIT (V560G/D816V)

HGNC

KIT (V560G/D816V)

Synonym

C-Kit, PBT, CD117, SCFR,

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.4
PP2 6.9
AG1478 2,000
PDGFR I-III 15
D-64406 500
Scientific Information

P10721 (http://www.uniprot.org/uniprot/P10721)

Screening Location

Malvern, USA

Reference compound IC50 for c-KIT (V560G/D816V)

Reference compound IC50 for c-KIT (V560G/D816V)