c-MET-KIF5B (Kex24Mex14) Kinase Assay Service

Activity-based c-MET-KIF5B (Kex24Mex14) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

c-MET, KIF5B (Kex24Mex14)-

Enzyme

Human KIF5B-c-MET (Kex24Mex14) Fusion protein

HGNC

MET-KIF5B

Synonym

KIF5B: Kinesin-1 heavy chain, Conventional kinesin heavy chain, KNS, KNS1
c-MET: Hepatocyte growth factor receptor, HGF receptor, Scatter factor receptor, SF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, c-Met, MET

Scientific Information

KIF5B: P33176 (http://www.uniprot.org/uniprot/P33176)
c-MET: P08581 (http://www.uniprot.org/uniprot/P08581)

Assay Properties
Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 22
PP2 >100,000
AG1478 17,000
EGFR/Erb Inhibitor >100,000
D-64406 14,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for c-MET-KIF5B (Kex24Mex14)