c-MET (G1163R) Kinase Assay Service

Activity-based c-MET (G1163R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-MET (G1163R)
Enzyme
Human c-MET (G1163R)
HGNC
MET (G1163R)
Synonym
c-MET, HGFR, RCCP2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine2,100
PP2>100,000
AG147811,000
EGFR/Erb Inhibitor>100,000
D-6440642,000
Scientific Information
P08581 (http://www.uniprot.org/uniprot/P08581)
Screening Location
Malvern, USA
Reference compound IC50 for C-MET (G1163R)
Reference compound IC50 for C-MET (G1163R)