c-MET Kinase Assay Service

Activity-based c-MET kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

c-MET

Enzyme

Human c-MET

HGNC

MET

Synonym

Hepatocyte growth factor receptor, HGF receptor, Scatter factor receptor, SF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, c-Met, MET

Scientific Information

P08581 (http://www.uniprot.org/uniprot/P08581)

Assay Properties
Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 34
PP2 >100,000
AG1478 8,100
EGFR/Erb Inhibitor >100,000
D-64406 11,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for c-MET