c-MET Kinase Mutant Profiler Service

Activity-based c-MET mutant kinase panel for compound profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

c-MET

Enzyme

Human c-MET

HGNC

MET

Synonym

Hepatocyte growth factor receptor [Precursor], HGF receptor, Scatter factor receptor, SF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, c-Met, MET

Assay Properties

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s (nM)
Mutant Staurosporine PP2 AG1478 PDGFR I-III EGFR/ErbB Inhibitor D-64406
D1228A 24 >100,000 21,500 n.a. >100,000 12,000
D1228G 32 >100,000 33,000 n.a. >100,000 10,000
D1228H 27 >100,000 17,000 n.a. >100,000 9,300
D1228N 24 >100,000 26,500 n.a. >100,000 9,900
D1228V 12 >100,000 20,500 n.a. >100,000 11,000
D1228Y 29 >100,000 52,000 n.a. >100,000 47,000
DelEx14 22 >100,000 21,000 n.a. >100,000 15,000
F1200I 34 >100,000 >100,000 n.a. >100,000 13,000
G1163R 590 >100,000 32,000 n.a. >100,000 75,000
H1094L 49 >100,000 >100,000 n.a. >100,000 20,000
H1094Y 70 >100,000 >100,000 n.a. >100,000 8,800
K1244R 21 >100,000 20,000 n.a. >100,000 16,000
KIF5B 22 >100,000 17,000 n.a. >100,000 14,000
L1195F 8.3 >100,000 16,000 n.a. >100,000 9,300
L1195V 31 >100,000 66,000 n.a. >100,000 9,300
M1250I 25 >100,000 19,000 n.a. >100,000 9,700
M1250T 59 >100,000 41,000 n.a. >100,000 15,000
P991S 24 >100,000 23,000 n.a. >100,000 17,000
R970C 20 >100,000 17,000 n.a. >100,000 18,000
T1173I 21 >100,000 25,000 n.a. >100,000 15,000
T992I 27 >100,000 26,000 n.a. >100,000 17,000
TFG 38 >100,000 16,000 n.a. >100,000 13,000
V1092I 50 >100,000 24,000 n.a. >100,000 40,000
WT 34 >100,000 8,100 n.a. >100,000 11,000
Y1230A 25 >100,000 50,000 n.a. >100,000 14,000
Y1230C 26 >100,000 27,000 n.a. >100,000 15,000
Y1230D 13 >100,000 17,000 n.a. >100,000 12,000
Y1230H 22 >100,000 39,000 n.a. >100,000 17,000
Y1230S 25 >100,000 >100,000 n.a. >100,000 18,000
Y1235D 40 >100,000 16,000 n.a. >100,000 21,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays