c-MET (M1250I) Kinase Assay Service

Activity-based c-MET (M1250I) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-MET (M1250I)
Enzyme
Human c-MET (M1250I)
HGNC
MET (M1250I)
Synonym
c-MET, HGFR, RCCP2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine250
PP2>100,000
AG147813,500
EGFR/Erb Inhibitor>100,000
D-6440617,000
Scientific Information
P08581 (http://www.uniprot.org/uniprot/P08581)
Screening Location
Malvern, USA
Reference compound IC50 for c-MET (M1250I)
Reference compound IC50 for c-MET (M1250I)
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