c-MET (P991S) Kinase Assay Service

Activity-based c-MET (P991S) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-MET (P991S)
Enzyme
Human c-MET (P991S)
HGNC
MET (P991S)
Synonym
c-MET, HGFR, RCCP2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine240
PP2>100,000
AG1478>100,000
PDGFR I-III>100,000
D-6440620,000
Scientific Information
P08581 (http://www.uniprot.org/uniprot/P08581)
Screening Location
Malvern, USA
Reference compound IC50 for C-MET (P991S)
Reference compound IC50 for C-MET (P991S)