c-SRC N1 Kinase Assay Service

Activity-based c-SRC kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

c-SRC N1

Enzyme

Human c-SRC N1

HGNC

SRC

Synonym

ASV, SRC1, c-SRC, p60-Src

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P12931 (http://www.uniprot.org/uniprot/P12931)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound inhibition of c-SRC N1