CAMK1b Kinase Assay Service

Activity-based CAMK1b kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CAMK1β
Enzyme
Human CAMK1β
HGNC
PNCK
Synonym
CAMK1b, MGC45419
Substrates
Peptide substrate, [KKALRRQETVDAL], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine11
Ro 31-822011,000
GW 507422,000
Rottlerin>50,000
Scientific Information
Q6P2M8 (http://www.uniprot.org/uniprot/Q6P2M8)
Screening Location
Malvern, USA
Reference compound IC50 for CAMK1β
Reference compound IC50 for CAMK1β