Activity-based CAMKK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CAMKK2
Enzyme
Human CAMKK2
HGNC
CAMKK2
Synonym
CAMKK, CAMKKB, KIAA0787, MGC15254
Substrates
MBP (Myelin Basic Protein), 10 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q96RR4 (http://www.uniprot.org/uniprot/Q96RR4)
Screening Location
Malvern, USA
Reference compound IC50 for CAMKK2
