Cholecystokinin CCK1 Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
CCK1 Binding
GPCR Target
Human CCK1
Source
Membrane preparation from 1321N1 cells stably transfected with human cholecystokinin CCK1 receptor
Scientific Information
CCKAR (https://www.uniprot.org/uniprotkb/P32238/entry)
Assay Properties
Protocol
Radioligand filter binding to CCK1 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Radioligand
CCK-8, 1 nM
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| Lorglumide | 168 |
| Devazepide | 1.14 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound binding data for CCK1
PDF