CDK12/CycK Kinase Activity Assay Service

Activity-based CDK12/CycK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

CDK12/CycK

Enzyme

Human CDK12/CycK, wt

HGNC

CDK12-CCNK

Manning ID

CDK10

Synonym

CRKRS, CRK7, KIAA0904, CDK12, CDK12 wt/CycK, CDK12/CycK, CDK12/cyclin K

UniProt

Q9NYV4 (CDK12_HUMAN)

Kinase Type

Protein kinase

Kinase Group

CMGC

Assay Properties

Assay Format

33PanQinaseTM

Substrates

RBER-IRStide, ATP

Reaction

RBER-IRStide + 33P-ATP = 33P-RBER-IRStide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 110
Sorafenib 10000
Sunitinb 38000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice CDK12/CycK
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 CDK Profiler
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – CDK12/CycK
(Activity verified by 33PanQinaseTM-Assay)
Product Number 1483-1484-1
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