CDK12 (R722C)/cyclin K Kinase Assay Service

Activity-based CDK12 (R722C)/cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK12 (R722C)/Cyclin K
Enzyme
Human CDK12 (R722C)/Cyclin K
HGNC
CDK12 (R722C)/CCNK
Synonym
CRKRS, CRK7
Substrates
Protein substrate, Pol2-CTD, 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
CDK12: Q9NYV4 (http://www.uniprot.org/uniprot/Q9NYV4)Cyclin K: O75909 (http://www.uniprot.org/uniprot/O75909)
Screening Location
Malvern, USA
Reference compound IC50 for CDK12 (R722C)/cyclin K