CDK18/cyclin Y Kinase Assay Service

Activity-based CDK18/cyclin Y kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK18/Cyclin Y
Enzyme
Human CDK18/Cyclin Y
HGNC
CDK18/CCNY
Synonym
PCTAIRE, PCTAIRE3, PCTK3
Substrates
RB protein, 3 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine27
Ro 31-82201.8
GW 507427,000
H-898,000
Rottlerin21,000
Scientific Information
CDK18: Q07002 (http://www.uniprot.org/uniprot/Q07002)Cyclin Y: Q8ND76 (http://www.uniprot.org/uniprot/Q8ND76)
Screening Location
Malvern, USA
Reference compound IC50 for CDK18/cyclin Y (PCTK3)
Reference compound IC50 for CDK18/cyclin Y (PCTK3)