CDK19/Cyclin C Kinase Assay Service

Activity-based CDK19/Cyclin C kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK19/Cyclin C

Enzyme

Human CDK19 (full length); Cyclin C (full length); MED12 (aa 1-100) complex

HGNC

CDK19

Synonym

CDK19: CDC2L6, CDK11, KIAA1028
Cyclin C: CCNC, SRB11 homolog (hSRB11)
MED12: ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230

Substrates

Protein substrate, Pol2-CTD, 5 µM
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	125
BI-1347	17
BRD6989	18.8
CCT251921	31.9

Scientific Information

CDK19: Q9BWU1 (http://www.uniprot.org/uniprot/Q9BWU1)
Cyclin C: P24863 (http://www.uniprot.org/uniprot/P24863)
MED12: Q93074 (http://www.uniprot.org/uniprot/Q93074)

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Kinase Screening Assay Services.

Reference compound inhibition for CDK19/Cyclin C