Activity-based CDK1/cyclin B kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK1/cyclinB
Enzyme
Human CDK1/cyclinB
HGNC
CDK1/CCNB1
Synonym
CDC2, p34
Substrates
RB Protein, 3 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.3 |
| Ro 31-8220 | 6.9 |
| GW 5074 | >100,000 |
| H-89 | 18,000 |
| Rottlerin | >100,000 |
Scientific Information
P06493 (http://www.uniprot.org/uniprot/P06493)
Screening Location
Malvern, USA
