Activity-based CDK2/CycO kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK2/CycO
Enzyme
Human CDK2, wt
HGNC
CDK2
Manning ID
CDK2
Synonym
CDK2, p33
UniProt
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
SUPT5H, ATP
Reaction
Substrate + 33P-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 8 |
| Sorafenib | 70000 |
| Sunitinib | 90000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK2/CycO |
| wt Profiler Panel | Yes |
| IC50 wt Profiler Panel | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK2/CycO (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0050-2135-1 |
| Web Shop | Reaction Biology Online Shop |
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