CDK2/cyclin E Kinase Assay Service

Activity-based CDK2/cyclin E kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK2/cyclin E

Enzyme

Human CDK2/cyclin E

HGNC

CDK2/CCNE1

Synonym

p33

Substrates

RB protein, 3µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.4
Ro 31-8220 5.0
GW5074 >100,000
H-89 4,300
Rottlerin >100,000
Scientific Information

CDK2: P24941 (http://www.uniprot.org/uniprot/P24941)Cyclin E: P24864 (http://www.uniprot.org/uniprot/P24864)

Screening Location

Malvern, USA

Reference compound IC50 for CDK2/cyclin E

Reference compound IC50 for CDK2/cyclin E