Activity-based CDK3/Cyclin E2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK3/Cyclin E2
Enzyme
Human CDK3/Cyclin E2
HGNC
CDK3/CCNE2
Synonym
CDK3: Cell division protein kinase 3, Cyclin-dependent kinase 3
Cyclin E2:G1/S-specific cyclin-E2, CCNE2
Substrates
RB Protein, 3µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.6 |
| Ro 31-8220 | 2.6 |
| GW5074 | 31,000 |
| H-89 | 6,200 |
| Rottlerin | >100,000 |
Scientific Information
CDK3:Q00526 (https://www.uniprot.org/uniprot/Q00526)
Cyclin E2:O96020 (http://www.uniprot.org/uniprot/O96020)
Screening Location
Malvern, USA
