CDK3/cyclin E Kinase Assay Service

Activity-based CDK3/cyclin E kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK3/Cyclin E

Enzyme

Human CDK3/Cyclin E

HGNC

CDK3/CCNE1

Synonym

CDKN3

Substrates

RB Protein, 3 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 2.5
Ro 31-8220 2.3
GW 5074 17,000
H-89 5,100
Rottlerin >100,000
Scientific Information

CDK3: Q00526 (http://www.uniprot.org/uniprot/Q00526)Cyclin E1: P24864 (http://www.uniprot.org/uniprot/P24864)

Screening Location

Malvern, USA

Reference compound IC50 for CDK3/Cyclin E