CDK3/cyclin E Kinase Assay Service

Activity-based CDK3/cyclin E kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK3/Cyclin E

Enzyme

Human CDK3/Cyclin E

HGNC

CDK3/CCNE1

Synonym

CDKN3

Substrates

RB Protein, 3 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK3: Q00526 (http://www.uniprot.org/uniprot/Q00526)Cyclin E1: P24864 (http://www.uniprot.org/uniprot/P24864)

Screening Location

Malvern, USA