CDK4/CycD1 Kinase Activity Assay Service

Activity-based CDK4/CycD1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

CDK4/CycD1

Enzyme

Human CDK4/CycD1, wt

HGNC

CDK4-CCND1

Manning ID

CDK4

Synonym

CMM3, PSK-J3, MGC14458, CDK4, CDK4/CycD1, CDK4/cyclin D1

UniProt

P11802 (CDK4_HUMAN)

Kinase Type

Protein kinase

Kinase Group

CMGC

Assay Properties

Assay Format

33PanQinaseTM

Substrates

RBER-CHKtide, ATP

Reaction

RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 46
Sorafenib 10000
Sunitinb 10000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice CDK4/CycD1
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 CDK Profiler
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – CDK4/CycD1
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0142-0143-1
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