CDK4/CycD2 Kinase Activity Assay Service

Activity-based CDK4/CycD2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

CDK4/CycD2

Enzyme

Human CDK4/CycD2, wt

HGNC

CDK4-CCND2

Manning ID

CDK4

Synonym

CMM3, PSK-J3, MGC14458, CDK4, CDK4/CycD2

UniProt

P11802 (CDK4_HUMAN)

Kinase Type

Protein kinase

Kinase Group

CMGC

Assay Properties
Assay Format

33PanQinaseTM

Substrates

RBER-CHKtide, ATP

Reaction

RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 105
Sorafenib 10000
Sunitinib 19000
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice CDK4/CycD2
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 CDK Profiler
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – CDK4/CycD2
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0142-0375-1
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