Activity-based CDK4/CycD2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK4/CycD2
Enzyme
Human CDK4/CycD2, wt
HGNC
CDK4-CCND2
Manning ID
CDK4
Synonym
CMM3, PSK-J3, MGC14458, CDK4, CDK4/CycD2
UniProt
P11802 (CDK4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
Compounds | IC50 (nM) |
---|---|
Staurosporine | 105 |
Sorafenib | 10000 |
Sunitinib | 19000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
FreeChoice | CDK4/CycD2 |
355 wt Profiler | Yes |
355 IC50 wt Profiler | Yes |
Mutant Profiler Addon | No |
Lipid Kinase Profiler Addon | No |
IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
Active recombinant protein | Yes; ProQinaseTM – CDK4/CycD2 (Activity verified by 33PanQinaseTM-Assay) |
Product Number | 0142-0375-1 |
Web Shop | Reaction Biology Online Shop |
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